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  • 匿名
关注:1 2013-05-23 12:21

求翻译:此外,由于GFP荧光蛋白本身存在假阳性,不能对基因的表达进行精确定位,因此也不能确切的说明SPS基因在叶绿体中有表达。如需进一步验证,还需要做免疫胶体金或原位杂交进行精确定位。是什么意思?

待解决 悬赏分:1 - 离问题结束还有
此外,由于GFP荧光蛋白本身存在假阳性,不能对基因的表达进行精确定位,因此也不能确切的说明SPS基因在叶绿体中有表达。如需进一步验证,还需要做免疫胶体金或原位杂交进行精确定位。
问题补充:

  • 匿名
2013-05-23 12:21:38
In addition, since the gfp fluorescent protein false positive, and not the expression of genes for accurate positioning, and therefore can not be precisely the sps gene expression in chloroplasts. For further validation, needs to be done to the immune colloidal gold or in situ hybridization for prec
  • 匿名
2013-05-23 12:23:18
In addition, as a result there is GFP fluorescent protein itself cannot be false positive, and the expression of genes for precise positioning, and therefore cannot be precise instructions in SPS genes in chloroplasts. If you need further validation, you also need to be immunized with colloidal gold
  • 匿名
2013-05-23 12:24:58
In addition, because GFP fluorescence protein itself has the false positive, cannot carry on the pinpointing to the gene expression, therefore cannot the accurate explanation SPS gene have the expression in the chloroplast.If must further confirm, but also needs to make the immunity colloid gold or
  • 匿名
2013-05-23 12:26:38
In addition, due to false-positive GFP fluorescence protein itself, not on the expression of genes for precise positioning, so it can't exactly SPS gene expression in the chloroplast. For further verification, colloidal gold or in situ hybridization was needed for accurate positioning.
  • 匿名
2013-05-23 12:28:18
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