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关注：1
2013-05-23 12:21
求翻译：利用PCR以健康人淋巴细胞基因组为模板，用两对克隆引物，扩增出野生型c-kit基因片段和含D816V突变的c-kit基因片段，并分别克隆至pEGFP- C1中，送测序是什么意思？ 待解决
 悬赏分：1
- 离问题结束还有
利用PCR以健康人淋巴细胞基因组为模板，用两对克隆引物，扩增出野生型c-kit基因片段和含D816V突变的c-kit基因片段，并分别克隆至pEGFP- C1中，送测序
问题补充： |
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2013-05-23 12:21:38
Lymphocytes in healthy people by PCR genome as a template, with two pairs of cloning primers for the amplification of wild-type c-kit gene fragment and with D816V c-kit gene mutation fragments were cloned into pEGFP-C1, the send sequence
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2013-05-23 12:23:18
正在翻译，请等待...
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2013-05-23 12:24:58
正在翻译，请等待...
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2013-05-23 12:26:38
Using PCR in healthy human lymphocyte genome as a template, using two primers on a clone, amplification of wild type and c-Kit gene containing D816V mutation of c-Kit gene and cloning to pEGFP-C1 respectively, send sequence
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2013-05-23 12:28:18
Lymphocytes in healthy people by PCR genome as a template, with two pairs of cloning primers for the amplification of wild-type c-kit gene fragment and with D816V c-kit gene mutation fragments were cloned into pEGFP-C1, the send sequence
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