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  • 匿名
关注:1 2013-05-23 12:21

求翻译:分别在诱导后的24h和48h取肾脏和脾脏,通过黄鳝总RNA的提取,cDNA第一链的反转录,IRF-4、IRF10的基因克隆,β-actin的基因克隆,最后半定量检测IRF-4、IRF-10的诱导表达。是什么意思?

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分别在诱导后的24h和48h取肾脏和脾脏,通过黄鳝总RNA的提取,cDNA第一链的反转录,IRF-4、IRF10的基因克隆,β-actin的基因克隆,最后半定量检测IRF-4、IRF-10的诱导表达。
问题补充:

  • 匿名
2013-05-23 12:21:38
24h and 48h after the induction to take the kidney and spleen, eel total rna extraction, reverse transcription of the first chain of cDNA for irf-4, irf10 Gene cloning, beta-actin gene cloning, the final semi-quantitative detection of irf- irf-10 induced expression.
  • 匿名
2013-05-23 12:23:18
After the induction of the 24H and 48 h, the kidney and spleen through the protective mother eel cDNA RNA extract, the first link of the antiretroviral drugs, IRF IRF 10 - 4, the gene cloning, β-actin gene cloning, and the final semi-quantitative test IRF IRF - 4 - 10, the induced expression.
  • 匿名
2013-05-23 12:24:58
Separately takes the kidney and the spleen after induction 24h and 48h, through eel total RNA extraction, cDNA first chain counter-duplication, IRF-4, IRF10 gene clone, β-actin the gene clone, finally semi-quantitative examines IRF-4, the IRF-10 induction expression.
  • 匿名
2013-05-23 12:26:38
Respectively in the induction and 24h takes after kidney and spleen, through total RNA extraction of monopterus Albus, cDNA reverse transcription of the first chain, IRF-4, IRF10, gene cloning, gene cloning of beta-actin, induces expression of the last semi-quantitative detection of IRF-4 and IRF-10
  • 匿名
2013-05-23 12:28:18
正在翻译,请等待...
 
 
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