求翻译：甲烷是重要的化工原料和化学能源，在人类的生产生活中扮演着重要的角色，而甲烷氧化菌的生存需要氧且以甲烷作为唯一碳源和能源进行代谢，其关键酶之一是甲烷单加氧酶。因此，对甲烷单加氧酶的基因工程研究有助于研究甲烷氧化菌对于甲烷的代谢机制。本实验对已有的甲烷氧化菌C3进行活化培养，然后对活化后的甲烷氧化菌C3进行纯化，从纯化后的C3菌中提取出甲烷单加氧酶的目的基因组，经过PCR扩增仪进行克隆，然后测序。是什么意思？

Methane is an important chemical raw materials and chemical energy, plays an important role in the production of human life, and survival of methanotrophs need oxygen and methane as sole carbon and energy metabolism, one of the key enzyme methane monooxygenase. Therefore, the genetic engineering of

Methane is an important raw material for the chemical industry and chemical energy in the human production, and in the life of the play an important role to play, and the methane bacteria carbon dioxide and oxygen needs of survival to methane as the only PHAs and energy metabolism, and the key enzym