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关注：1
2013-05-23 12:21
求翻译：The OD595 was determined for baseline optical density. The cultures were then expelled from the plate, which was washed twice with 200 l of distilled water per well. The plates were allowed to dry, and then the cells were fixed at 80°C for 30 min. Following staining with 0.1% crystal violet for 20 min, the wells were 是什么意思？ 待解决
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- 离问题结束还有
The OD595 was determined for baseline optical density. The cultures were then expelled from the plate, which was washed twice with 200 l of distilled water per well. The plates were allowed to dry, and then the cells were fixed at 80°C for 30 min. Following staining with 0.1% crystal violet for 20 min, the wells were
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2013-05-23 12:21:38
od595被确定为基准的光密度。从板，这是蒸馏水200 l每洗两次，然后被驱逐的文化。板被允许干，然后将细胞固定在80掳C的30分钟。 20分钟的0.1％结晶紫染色后，井用200升95％乙醇脱色。
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2013-05-23 12:23:18
正在翻译，请等待...
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2013-05-23 12:24:58
OD595为基础线光学密度是坚定的。 文化从板材然后被逐出了，两次洗涤与200 蒸馏水l每口井。 板材让烘干，然后细胞是固定的在80°C 30 min。 跟随的弄脏与0.1%结晶紫20分钟，井是destained与200 l 95%对氨基苯甲酸二。
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2013-05-23 12:26:38
OD595 确定基准光密度。然后，文化被驱逐出车牌的两次与每井蒸馏水的 200 l 被冲走。盘子获准以干，然后单元格被固定在 80 ° C，30 分钟。后 20 分钟，0.1%结晶紫染色，井被 destained 200 l 95%乙醇。
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2013-05-23 12:28:18
OD595 为底线被确定光学密度。The cultures were then expelled from the plate, which was washed twice with 200 l of distilled water per well.盘子被允许变干，然后细胞持续 30 分钟在 80 ° C 被固定。Following staining with 0.1% crystal violet for 20 min, the wells were destained with 200 l 95% ethanol.
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