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  • 匿名
关注:1 2013-05-23 12:21

求翻译:根据大量实验选择出RAPD引物10条,SSR引物8对扩增条带稳定、清晰、重复性好、多态性高的引物,在2份供试品种间进行PCR扩增。采用琼脂糖凝胶电泳和非变性聚丙烯酰胺凝胶电泳后检测,RAPD引物共扩增出90条带, 其中44条带具有多态性,多态性为48.9%;SSR引物共扩增出133条带,其中84条带具有多态性,多态性为63.2%,是什么意思?

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根据大量实验选择出RAPD引物10条,SSR引物8对扩增条带稳定、清晰、重复性好、多态性高的引物,在2份供试品种间进行PCR扩增。采用琼脂糖凝胶电泳和非变性聚丙烯酰胺凝胶电泳后检测,RAPD引物共扩增出90条带, 其中44条带具有多态性,多态性为48.9%;SSR引物共扩增出133条带,其中84条带具有多态性,多态性为63.2%,
问题补充:

  • 匿名
2013-05-23 12:21:38
A large number of experiments to select rapd primers 10 ssr primers amplified bands were stable, clear, good repeatability, high polymorphism primer pcr amplification, between the two varieties tested. Detected after agarose gel electrophoresis and non-denaturing polyacrylamide gel electrophoresis r
  • 匿名
2013-05-23 12:23:18
Based on extensive lab lead the RAPD SSR article 10, 8 lead to an expansion of a stable, clear, repetitive, and multi-state, high in the lead, 2 species for resources for PCR escalations. In agar sugar gel electrophoresis and the non-transsexual poly-acrylamide gel electrophoresis detected after RAP
  • 匿名
2013-05-23 12:24:58
Chooses RAPD according to the massive experiments to direct thing 10, SSR to direct the thing 8 pairs to expand increases the banding stably, clear, the duplication good, the polymorphism high directs the thing, for tries the variety in 2 to carry on PCR to expand increases.After uses the agarose ge
  • 匿名
2013-05-23 12:26:38
Select 10 RAPD primers based on a large number of experiments, and 8 SSR primers for amplification with a stable, clear, high repeatability, and polymorphism of primer, 2 for PCR amplification for variety. Using agarose gel electrophoresis and detection by non-denaturing polyacrylamide gel electroph
  • 匿名
2013-05-23 12:28:18
 
 
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