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关注：1
2013-05-23 12:21
求翻译：The results were completely different with protoplasts obtained from the CS3 plants. HsfA1 was not expressed at all, even when protoplasts were transformed with 10μg (Fig. 5B, CS3) or 20 μg (data not shown) of the HsfA1 encoding plasmid. As a consequence, there was no detectable increase of GUS expression above the bac是什么意思？ 待解决
 悬赏分：1
- 离问题结束还有
The results were completely different with protoplasts obtained from the CS3 plants. HsfA1 was not expressed at all, even when protoplasts were transformed with 10μg (Fig. 5B, CS3) or 20 μg (data not shown) of the HsfA1 encoding plasmid. As a consequence, there was no detectable increase of GUS expression above the bac
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2013-05-23 12:21:38
结果与从CS3植物中获得的原生质体完全不同。 hsfa1不表达，即使10μg的（图5b，CS3）或20微克hsfa1编码质粒（数据未显示）原生质体转化。作为一个后果，有没有GUS表达上述背景探测增加。相比之下，与hsfa2表达质粒（兴建7）转化的样品给了GUS活性明显增加（图5b，CS3）和一个强大的hsfa2对免疫（图5C，CS3）的信号。嵌合蛋白的表达水平和激活功能依赖于混合结构（图5A）在hsfa1部分。两个主要部分的hsfa1（结构2和3）嵌合HSFS表达了强烈的沉默，而次要部分hsfa1两种蛋白质表达的影响较小。从图3所示的结果预期，这是明显的沉默效果是否5_部分（结构2和5），3_
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2013-05-23 12:23:18
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2013-05-23 12:24:58
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2013-05-23 12:26:38
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2013-05-23 12:28:18
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