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关注：1
2013-05-23 12:21
求翻译：采用RT-PCR方法,利用牛病毒性腹泻病毒(BVDV)C24V株接种MDBK细胞,提取病毒RNA，扩增出BVDV-E0基因,与pET28a原核表达载体分别经BamHⅠ和HandIII双酶切,连接,经鉴定后证明成功构建pET28a-E0原核表达载体,经IPTG诱导通过SDS-PAGE和Western-blot检测到目的蛋白表达,为研究蛋白功能和单克隆抗体的研制打下了基础。是什么意思？ 待解决
 悬赏分：1
- 离问题结束还有
采用RT-PCR方法,利用牛病毒性腹泻病毒(BVDV)C24V株接种MDBK细胞,提取病毒RNA，扩增出BVDV-E0基因,与pET28a原核表达载体分别经BamHⅠ和HandIII双酶切,连接,经鉴定后证明成功构建pET28a-E0原核表达载体,经IPTG诱导通过SDS-PAGE和Western-blot检测到目的蛋白表达,为研究蛋白功能和单克隆抗体的研制打下了基础。
问题补充： |
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2013-05-23 12:21:38
By RT-PCR method, the use of bovine viral diarrhea virus (BVDV) C24V strains of MDBK cells inoculated, extracted viral RNA, BVDV-E0 gene was amplified with prokaryotic expression vector pET28a, respectively, by BamH Ⅰ and HandIII restriction enzyme digestion, ligation, by After identification proved
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2013-05-23 12:23:18
正在翻译，请等待...
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2013-05-23 12:24:58
正在翻译，请等待...
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2013-05-23 12:26:38
正在翻译，请等待...
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2013-05-23 12:28:18
Adopt RT-PCR method, utilize the viral diarrhoea and virus (BVDV) C24V one of ox to inoculate MDBK cells, withdraws virus RNA, amplify out BVDV-E0 gene, express with pET28a originally and nuclearly carriers are cut by one pair of enzymes BamHⅠ and HandIII separately, connects, prove that succeeds in
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