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关注：1
2013-05-23 12:21
求翻译：The supernatants were then transferred to new tubes and after adding equal volume of –20°C isopropanol and keeping at -20°C for 30 min, the samples were centrifuged at 12000 rpm for 10 min and the supernatants were discarded. Finally 250 μl of 4°C ethanol (70%) were added and the samples centrifuged at 10000 rpm for 5 是什么意思？ 待解决
 悬赏分：1
- 离问题结束还有
The supernatants were then transferred to new tubes and after adding equal volume of –20°C isopropanol and keeping at -20°C for 30 min, the samples were centrifuged at 12000 rpm for 10 min and the supernatants were discarded. Finally 250 μl of 4°C ethanol (70%) were added and the samples centrifuged at 10000 rpm for 5
问题补充： |
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2013-05-23 12:21:38
上清转移到新管，加入等体积-20℃异丙醇，在-20℃保持30分钟后，样品离心10分钟的转速在12000，上清液被丢弃。最后250 ° C乙醇（70％）4μL分别加入样品离心5分钟10000转。
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2013-05-23 12:23:18
然后被转到新的supernatants管和之后的平等量-20°C-20°C异丙醇和维持和平在为30分钟,样本的centrifuged在12000RPM10min和supernatants被丢弃。 最后250μl4°C乙醇(70%)和增加了的样本在10000RPM centrifuged为5分钟
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2013-05-23 12:24:58
正在翻译，请等待...
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2013-05-23 12:26:38
上清再迁至新管和添加相同的音量-20 ° C 异丙醇和保持在-20 ℃ 30 分钟之后, 样品被离心为 12000 rpm 10 分钟上, 清被丢弃。最后添加了 250 μl 的 4 ° C 乙醇 （70%） 和样本离心为 10000 rpm 5 分钟。
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2013-05-23 12:28:18
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