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关注：1
2013-05-23 12:21
求翻译：并将连接产物转化到氯化钙法制备的感受态细胞中，涂布于含有X—Gal和IPTG的LB培养平板中，经37℃培养12—16h后，是什么意思？ 待解决
 悬赏分：1
- 离问题结束还有
并将连接产物转化到氯化钙法制备的感受态细胞中，涂布于含有X—Gal和IPTG的LB培养平板中，经37℃培养12—16h后，
问题补充： |
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2013-05-23 12:21:38
And the ligation product was transformed into calcium chloride competent cells prepared, coated on the plates containing X-gal and iptg of LB culture, culture after 12-16h by 37 ℃
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2013-05-23 12:23:18
It will be connected into a calcium chloride product rule mentality back the feelings in the cells, coated in X - containing the LB IPTG Gal and flat building, in the 37 - 12 °C training after 16 h.
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2013-05-23 12:24:58
And will connect the product to transform to in the calcium chloride law preparation feeling condition cell, will spread the cloth in to include X-Gal and in the IPTG LB raise plate, after 37℃ will raise 12-16h,
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2013-05-23 12:26:38
And connection products into to the preparation of calcium chloride competent cells, coated in LB in a culture plate containing IPTG and X-Gal, 37 ° c after train 12-16h,
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2013-05-23 12:28:18
正在翻译，请等待...
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