求翻译：所得上清液依次经过截留分子量10000中空纤维柱浓缩，85%硫酸铵沉淀，然后经过DEAE-Sepharose F.F.离子柱和Phenyl Sepharose CL-4B柱进行交换层析和疏水层析，是什么意思？

The resultant supernatant was sequentially through the hollow fiber column MWCO 10000 concentrated to 85% ammonium sulfate precipitation, and then through ion column of DEAE-Sepharose FF and phenyl sepharose cl-4b column exchange chromatography and hydrophobic interaction chromatography,

The Shangqing fluid trapped in the molecular weight 10,000 hollow-fiber post enrichment, 85% ammonium sulfate precipitation, and then after a DEAE - Sepharose F F . . ion column and Phenyl Sepharose B CL - 4 column in the Exchange layer parse layer parse and drain.

正在翻译，请等待...

Supernatant obtained after interception by molecular weight 10,000 hollow fibre post enrichment in turn, 85%, ammonium sulfate precipitation, and then through DEAE-Sepharose F.F. ion exchange chromatography and Phenyl Sepharose column CL-4B column and hydrophobic,

The obtained supernate passes through in turn the interception molecular weight 10000 spatial textile fiber column concentration, 85% ammonium sulfate precipitate, then ion column and Phenyl Sepharose the CL-4B column carries on the exchange chromatographic analysis and the sparse water chromatograp