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关注:1 2013-05-23 12:21

求翻译:mM dNTPs, 0.2 M “outer” primers, 5 l of RT product and 0.2 l of platinum TaqDNA polymerase (Invitrogen). The protocol included an initial 5 min denaturizing step at 95°C followed by 40 cycles of 40 s denaturation at 95°C, 40 s annealing at 55°C, and 40 s elongation at 72°C. The reaction was completed with 7 min of f是什么意思?

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mM dNTPs, 0.2 M “outer” primers, 5 l of RT product and 0.2 l of platinum TaqDNA polymerase (Invitrogen). The protocol included an initial 5 min denaturizing step at 95°C followed by 40 cycles of 40 s denaturation at 95°C, 40 s annealing at 55°C, and 40 s elongation at 72°C. The reaction was completed with 7 min of f
问题补充:

  • 匿名
2013-05-23 12:21:38
毫米的dNTPs,0.2米的“外”引物,反转录产物的5升和0.2升铂Taq DNA聚合酶(Invitrogen公司)。该协议包括一个初始5分钟denaturizing的步骤在95℃时由40个循环40秒的变性,在95℃时,在55℃退
  • 匿名
2013-05-23 12:23:18
  • 匿名
2013-05-23 12:24:58
  • 匿名
2013-05-23 12:26:38
  • 匿名
2013-05-23 12:28:18
 
 
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