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关注:1
2013-05-23 12:21
求翻译:mM dNTPs, 0.2 M “outer” primers, 5 l of RT product and 0.2 l of platinum TaqDNA polymerase (Invitrogen). The protocol included an initial 5 min denaturizing step at 95°C followed by 40 cycles of 40 s denaturation at 95°C, 40 s annealing at 55°C, and 40 s elongation at 72°C. The reaction was completed with 7 min of f是什么意思? 待解决
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mM dNTPs, 0.2 M “outer” primers, 5 l of RT product and 0.2 l of platinum TaqDNA polymerase (Invitrogen). The protocol included an initial 5 min denaturizing step at 95°C followed by 40 cycles of 40 s denaturation at 95°C, 40 s annealing at 55°C, and 40 s elongation at 72°C. The reaction was completed with 7 min of f
问题补充: |
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