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关注：1
2013-05-23 12:21
求翻译：Single-cell reverse-transcription PCR (RTPCR). Single-cell RT-PCR was performed as previously described (Park et al., 2006; Liu et al., 2010). Briefly, following whole-cell patchclamp recordings, spinal lamina II neurons or DRG neurons were harvested into patch pipettes with tip diameters of 15–25 m, gently put into r是什么意思？ 待解决
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Single-cell reverse-transcription PCR (RTPCR). Single-cell RT-PCR was performed as previously described (Park et al., 2006; Liu et al., 2010). Briefly, following whole-cell patchclamp recordings, spinal lamina II neurons or DRG neurons were harvested into patch pipettes with tip diameters of 15–25 m, gently put into r
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2013-05-23 12:21:38
单细胞的反转录聚合酶链反应（RT-PCR）。单细胞RT-PCR方法进行，如前所述（公园等，2006;刘等人，2010）。简单地说，全细胞patchclamp录音，脊髓Ⅱ板层神经元和背根神经节神经元收获到补丁移液器，尖直径为15-25米，轻轻地放入含有反转录试剂的反应管，在50℃保温1 h（标三，Invitrogen公司）。独立的PCR的cDNA产品中使用了。使用的引物序列示于表1。进行第一轮PCR在50 l的PCR缓冲液中含有0.2
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2013-05-23 12:23:18
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2013-05-23 12:24:58
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2013-05-23 12:26:38
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2013-05-23 12:28:18
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