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关注:1
2013-05-23 12:21
求翻译:The whole-cell patch-clamp recordings were made from outer lamina II (lamina IIo) neurons in oltage-clamp mode. Patch pipettes were fabricated from thin-walled, borosilicate, glass-capillary tubing (1.5 mm outer diameter, World Precision Instruments). After establishing the whole-cell configuration, neurons were held 是什么意思?![]() ![]() The whole-cell patch-clamp recordings were made from outer lamina II (lamina IIo) neurons in oltage-clamp mode. Patch pipettes were fabricated from thin-walled, borosilicate, glass-capillary tubing (1.5 mm outer diameter, World Precision Instruments). After establishing the whole-cell configuration, neurons were held
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2013-05-23 12:21:38
全细胞膜片钳记录由外层(层IIO)在oltage钳模式下的神经元。补丁的移液器制作从薄壁,高硼硅玻璃毛细管(1.5毫米外径,世界精密仪器)。建立整个单元配置后,神经元的可能性为70 mV的记录sepscs举行。一个典型的补丁移液器的电阻是5-10米。内部溶液含有(inmm):135葡萄糖酸钾,5 KCL,0.5氯化钙,氯化镁,5 EGTA,5 HEPES,5 ATP毫克。膜电流Axopatch 200B中放大器(分子器件),在电压钳模式下进行扩增。在2千赫的信号被过滤和数字化的5千赫。数据存储与个人电脑采用膜片钳pClamp 10软件和与微型分析(synaptosoft公司)分析。
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2013-05-23 12:23:18
整个细胞修补程序-锁模录像是从外叶片II(叶片内部调查课)神经元在电压-锁模模式。 修补程序从吸液管是捏造的薄壁、硼硅酸盐玻璃毛细管管(1.5mm外径、世界精密的仪器)。 建立后的整体单元配置中,举行了神经元的潜力在70mv sepscs记录。 电阻的一个典型的补丁程序吸液针
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2013-05-23 12:24:58
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2013-05-23 12:26:38
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2013-05-23 12:28:18
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